Experiment information
Accession CRX019677
Organism Homo sapiens
Title N1
BioProject PRJCA000536
BioSample SAMC016521
Platform Illumina HiSeq 2000
Library
Library name Construction protocol Strategy Source Selection Layout
Genomic DNA were isolated using the DNeasy Blood and Tissue Kit (QIAGEN) or QIAamp DNA FFPE Tissue Kit (QIAGEN).Exome capture was performed on 3-6 ?g of genomic DNA per sample with the Agilent SureSelect Human All Exon V4 kit according to the manufacturer’s instructions. In brief, genomic DNA were fragmented by ultrasonicator (Covaris) to obtain fragments mainly distributed between 250 and 300 bp in length. These fragments were amplified using a NEBNext Ultra DNA Library Prep Kit (NEB) and then hybridized to the Illumina TruSeq capture kit for enrichment. Each captured library was loaded on the Illumina Hiseq2000 platform, and paired-end sequencing was performed to the desired median sequencing depth (>200×). Raw image files were processed using HCS1.4.8 for base calling with default parameters. WXS GENOMIC Hybrid Selection PAIRED
Processing Planned read length (bp) for mate 1: 100
Planned read length (bp) for mate 2: 100
Insert size (bp): 250
Release date2018-08-20
Run
Run accession Run data file information
File nameFile size (MB)
CRR021416 CRR021416_f1.fq.gz
CRR021416_r2.fq.gz
3,968.7
3,999.61
CRR021417 CRR021417_f1.fq.gz
CRR021417_r2.fq.gz
5,015.02
4,949.24
SubmitterJingjing Li (jijilee_82@sina.com)
OrganizationBeijing cancer hospital
Date submitted2017-09-02