Accession | CRX019679 |
Organism | Homo sapiens |
Title | N5 |
BioProject | PRJCA000536 |
BioSample | SAMC016523 |
Platform | Illumina HiSeq 2000 |
Library |
Library name |
Construction protocol |
Strategy |
Source |
Selection |
Layout |
|
Genomic DNA were isolated using the DNeasy Blood and Tissue Kit (QIAGEN) or QIAamp DNA FFPE Tissue Kit (QIAGEN).Exome capture was performed on 3-6 ?g of genomic DNA per sample with the Agilent SureSelect Human All Exon V4 kit according to the manufacturer’s instructions. In brief, genomic DNA were fragmented by ultrasonicator (Covaris) to obtain fragments mainly distributed between 250 and 300 bp in length. These fragments were amplified using a NEBNext Ultra DNA Library Prep Kit (NEB) and then hybridized to the Illumina TruSeq capture kit for enrichment. Each captured library was loaded on the Illumina Hiseq2000 platform, and paired-end sequencing was performed to the desired median sequencing depth (>200×). Raw image files were processed using HCS1.4.8 for base calling with default parameters. |
WXS |
GENOMIC |
Hybrid Selection |
PAIRED
|
|
Processing |
Planned read length (bp) for mate 1: 100
Planned read length (bp) for mate 2: 100
Insert size (bp): 250
|
Release date | 2018-08-20 |
Run |
Run accession |
Run data file information |
File name | File size (MB) |
CRR021420 |
CRR021420_f1.fq.gz
CRR021420_r2.fq.gz
|
3,670.47
3,704.78
|
CRR021421 |
CRR021421_f1.fq.gz
CRR021421_r2.fq.gz
|
4,101.01
4,151.49
|
|
Submitter | Jingjing Li (jijilee_82@sina.com) |
Organization | Beijing cancer hospital |
Date submitted | 2017-09-02 |