Accession | CRX023500 |
Organism | Mus musculus |
Title | Skeletal muscle total RNA |
BioProject | PRJCA000751 |
BioSample | SAMC021008 |
Platform | Illumina HiSeq 2500 |
Library |
Library name |
Construction protocol |
Strategy |
Source |
Selection |
Layout |
|
Total RNA was isolated using TRIZOL (Invitrogen, Carlsbad, CA). The RNA concentration and quality were determined with NanoDrop, Qubit and Agilent 2100 instruments. A RiboMinus kit (KAPA, USA) was used to deplete ribosomal RNA and rRNA- samples were used as templates for separate cDNA libraries following the TruSeq protocol (Illumina, San Diego, CA). Finally, the library was sequenced on the Illumina HiSeq 2500 platform at the Research Facility Center at Beijing Institutes of Life Science, CAS, with paired-end 250 bp (PE250) kits. |
RNA-Seq |
TRANSCRIPTOMIC |
PCR |
PAIRED
|
|
Processing |
Planned read length (bp) for mate 1: 250
Planned read length (bp) for mate 2: 250
Insert size (bp): 500
|
Release date | 2019-01-20 |
Run |
Run accession |
Run data file information |
File name | File size (MB) |
CRR026075 |
CRR026075_f1.fq.gz
CRR026075_r2.fq.gz
|
4,780.64
5,755.68
|
|
Submitter | Peifeng Ji (jipeifeng@biols.ac.cn) |
Organization | Beijing Institutes of Life Science, Chinese Academy of Sciences |
Date submitted | 2018-02-08 |