Experiment information
Accession CRX023510
Organism Homo sapiens
Title Brain RNase R
BioProject PRJCA000751
BioSample SAMC021011
Platform Illumina HiSeq 2500
Library
Library name Construction protocol Strategy Source Selection Layout
Total RNA was isolated using TRIZOL (Invitrogen, Carlsbad, CA). The RNA concentration and quality were determined with NanoDrop, Qubit and Agilent 2100 instruments. A RiboMinus kit (KAPA, USA) was used to deplete ribosomal RNA and then was incubated at 37 °C with 10 U ?g-1 RNase R (Epicentre, Madison, WI). RNA-/RNAase R-treated samples were used as templates for separate cDNA libraries following the TruSeq protocol (Illumina, San Diego, CA).Finally, the library was sequenced on the Illumina HiSeq 2500 platform at the Research Facility Center at Beijing Institutes of Life Science, CAS, with paired-end 250 bp (PE250) kits. RNA-Seq TRANSCRIPTOMIC PCR PAIRED
Processing Planned read length (bp) for mate 1: 250
Planned read length (bp) for mate 2: 250
Insert size (bp): 500
Release date2019-01-20
Run
Run Accession Release date Run Data File Information
File nameFile size (MB)
CRR026085 2019-01-20 BrCe_1.fq.gz
BrCe_2.fq.gz
2,387.83
2,637.21
SubmitterPeifeng Ji  (jipeifeng@biols.ac.cn)
OrganizationsBeijing Institutes of Life Science, Chinese Academy of Sciences
Date submitted2018-02-08