Experiment information
Accession CRX023538
Organism Homo sapiens
Title Heart poly(A)
BioProject PRJCA000751
BioSample SAMC021020
Platform Illumina HiSeq 2500
Library
Library name Construction protocol Strategy Source Selection Layout
Total RNA was isolated using TRIZOL (Invitrogen, Carlsbad, CA). The RNA concentration and quality were determined with NanoDrop, Qubit and Agilent 2100 instruments. Total RNA was used to prepare a poly(A)-selected library following the TruSeq v2 guide.Finally, the poly(A)-selected libraries were sequenced on the Illumina HiSeq 2500 platform at the Research Facility Center at Beijing Institutes of Life Science, CAS, with paired-end 150 bp (PE250) kits. RNA-Seq TRANSCRIPTOMIC PCR PAIRED
Processing Planned read length (bp) for mate 1: 150
Planned read length (bp) for mate 2: 150
Insert size (bp): 300
Release date2019-01-20
Run
Run Accession Release date Run Data File Information
File nameFile size (MB)
CRR026113 2019-01-20 He_1.fq.gz
He_2.fq.gz
1,943.95
2,042.11
SubmitterPeifeng Ji  (jipeifeng@biols.ac.cn)
OrganizationsBeijing Institutes of Life Science, Chinese Academy of Sciences
Date submitted2018-02-08