Experiment information
Accession CRX019702
Organism Homo sapiens
Title Whole Genome Bisulfite Sequencing of NCSC
BioProject PRJCA000539
BioSample SAMC016542
Platform Illumina HiSeq 2000
Library
Library name Construction protocol Strategy Source Selection Layout
DNA of sorted NCSCs was extracted from the cell line using a QIAamp DNA Mini Kit (QIAGEN, Hilden, Germany), sheared to approximately 300-500 bp using a Covaris S220 instrument (Life Technologies, USA). Then the libraries were constructed through end-repair, A-tailing, adapter ligation and bisulfate-converted using a ZymoEZ DNA Methylation Kit (Zymo Research). Then it was sequenced on Illummia Hiseq 2000. Bisulfite-Seq GENOMIC unspecified PAIRED
Processing Planned read length (bp) for mate 1: 101
Planned read length (bp) for mate 2: 101
Insert size (bp): 400
Release date2019-09-04
Run
Run Accession Release date Run Data File Information
File nameFile size (MB)
CRR021471 2019-09-04 NCSC_WGBS_R1.fastq.gz
NCSC_WGBS_R2.fastq.gz
35,401.32
33,283.1
SubmitterKe An (anke@big.ac.cn)
OrganizationsBeijing Institute of Genomics, Chinese Academy of Sciences
Date submitted2017-09-04
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