| Accession | CRX019702 |
| Organism | Homo sapiens |
| Title | Whole Genome Bisulfite Sequencing of NCSC |
| BioProject | PRJCA000539 |
| BioSample | SAMC016542 |
| Platform | Illumina HiSeq 2000 |
| Library |
| Library name |
Construction protocol |
Strategy |
Source |
Selection |
Layout |
|
DNA of sorted NCSCs was extracted from the cell line using a QIAamp DNA Mini Kit (QIAGEN, Hilden, Germany), sheared to approximately 300-500 bp using a Covaris S220 instrument (Life Technologies, USA). Then the libraries were constructed through end-repair, A-tailing, adapter ligation and bisulfate-converted using a ZymoEZ DNA Methylation Kit (Zymo Research). Then it was sequenced on Illummia Hiseq 2000. |
Bisulfite-Seq |
GENOMIC |
unspecified |
PAIRED
|
|
| Processing |
Planned read length (bp) for mate 1: 101
Planned read length (bp) for mate 2: 101
Insert size (bp): 400
|
| Release date | 2019-09-04 |
|---|
| Run |
| Run accession |
Run data file information |
| File name | File size (MB) |
|---|
| CRR021471 |
CRR021471_f1.fastq.gz
CRR021471_r2.fastq.gz
|
35,401.32
33,283.1
|
|
| Submitter | Ke An (anke@big.ac.cn) |
|---|
| Organization | Beijing Institute of Genomics, Chinese Academy of Sciences |
|---|
| Date submitted | 2017-09-04 |
|---|
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