Experiment information
Accession CRX019705
Organism Homo sapiens
Title ChIP-Seq (H3K27me3) of CSC
BioProject PRJCA000539
BioSample SAMC016541
Platform Illumina HiSeq 3000
Library
Library name Construction protocol Strategy Source Selection Layout
ChIP experiments were performed according to the procedure described previously and used specific antibodies for H3K27me3 with minimal modification. Two independent biological replicates were set for CSCs. DNA fragments were purified with the QIAquick PCR purification kit (Qiagen 28106). Sequencing for H3K27me3 (paired-ending sequencing) was performed on the Illumina HiSeq3000 platform. ChIP-Seq GENOMIC ChIP PAIRED
Processing Planned read length (bp) for mate 1: 101
Planned read length (bp) for mate 2: 101
Insert size (bp): 400
Planned read length (bp): 101
Release date2019-09-04
Run
Run Accession Release date Run Data File Information
File nameFile size (MB)
CRR021482 2019-09-04 CSC_H3K27me3_BiologicalReplicate1_R1.fastq.gz
CSC_H3K27me3_BiologicalReplicate1_R2.fastq.gz
2,445.97
2,486.92
CRR021483 2019-09-04 CSC_H3K27me3_BiologicalReplicate2_R1.fastq.gz
CSC_H3K27me3_BiologicalReplicate2_R2.fastq.gz
2,869.34
2,911.02
CRR021484 2019-09-04 CSC_H3K27me3_Input_R1.fastq.gz
CSC_H3K27me3_Input_R2.fastq.gz
1,721.66
1,771.1
SubmitterKe An (anke@big.ac.cn)
OrganizationsBeijing Institute of Genomics, Chinese Academy of Sciences
Date submitted2017-09-04
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