Experiment information
Accession CRX020217
Organism Arabidopsis thaliana
Title Transcriptome of wild type and miR408 overexpressor in Arabidopsis
BioProject PRJCA000613
BioSample SAMC017083
Platform Illumina Genome Analyzer II
Library name Construction protocol Strategy Source Selection Layout
Total RNA was extracted with an RNeasy plant mini kit from the whole seedlings of wild type and miR408 transgenic line grown on MS for 10 days under continuous light in a growth chamber. The mRNA fraction was extracted from total RNA using Dynabeads oligo(dT)25. Double-strand cDNA were generated using Superscript II and random hexamer primers and fragmented by nebulization, and used for mRNA library construction. RNA-Seq TRANSCRIPTOMIC PCR FRAGMENT
Processing Planned read length (bp): 50
Release date2017-11-30
Run accession Release date Run data file information
File nameFile size (MB)
CRR022335 2017-11-30 MIR408-OX_sequence.fastq.gz 582.17
CRR022336 2017-11-30 WT_sequence.fastq.gz 439.65
SubmitterHUIYONG ZHANG (huiyong.zhang@henau.edu.cn)
OrganizationsHenan Agricultural University
Date submitted2017-10-31
Related experiments