Experiment information
Accession CRX021663
Organism Homo sapiens
Title ILMN11T
BioProject PRJCA000610
BioSample SAMC018538
Platform Illumina HiSeq 2000
Library
Library name Construction protocol Strategy Source Selection Layout
Human genomic DNA was extracted from frozen tumor or matched colon tissue with QIAampDNA Mini kits (Qiagen). Covaris system is used to obtain the fragmented genomic DNA between 150 to 200bp. Adaptors were ligated to both ends of the fragments and adaptor-ligated templates were then purified using AgencourtAMPure SPRI beads. Whole-exome enrichment was performed by the SureSelect Human All Exon 51M kit (Agilent). Captured DNA libraries are sequenced by HiSeq 2000 Genome Analyzer (Illumina). WXS GENOMIC PCR PAIRED
Processing Planned read length (bp) for mate 1: 90
Planned read length (bp) for mate 2: 90
Insert size (bp): 170
Release date2018-05-29
Run
Run Accession Release date Run Data File Information
File nameFile size (MB)
CRR023907 2018-05-29 ILMN11T.bam 12,804.6
SubmitterZhe Liu (liuzhebiosci@163.com)
OrganizationsAnzhen Hospital
Date submitted2017-12-01
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