实验基本信息
实验编号 CRX021751
物种名称 Homo sapiens
项目编号 PRJCA000610
样本编号 SAMC018626
测序平台 Complete Genomics
建库信息
文库名称 文库构建方法 建库策略 样品来源 实验选择 文库布局
Human genomic DNA was extracted from frozen tumor or matched colon tissue with QIAampDNA Mini kits (Qiagen). Covaris system is used to obtain the fragmented genomic DNA between 150 to 200bp. Adaptors were ligated to both ends of the fragments and adaptor-ligated templates were then purified using AgencourtAMPure SPRI beads. Whole-exome enrichment was performed by the SureSelect Human All Exon 51M kit (Agilent). Captured DNA libraries are sequenced by HiSeq 2000 Genome Analyzer (Illumina). WXS GENOMIC PCR PAIRED
处理信息 Planned read length (bp) for mate 1: 90
Planned read length (bp) for mate 2: 90
Insert size (bp): 170
发布日期2018-05-29
测序反应
Run编号 发布日期 Run序列文件信息
File nameFile size (MB)
CRR023995 2018-05-29 ILMN01N.bam 12,832.91
提交Zhe Liu (liuzhebiosci@163.com)
所属单位Anzhen Hospital
提交日期2017-12-01
关联实验
Experiments(195)