Experiment information
Accession CRX022283
Organism human gut metagenome
Title Healthy cohort sample 01 gut microbiota
BioProject PRJCA000673
BioSample SAMC019257
Platform Illumina HiSeq 2500
Library
Library name Construction protocol Strategy Source Selection Layout
A total of 200mg of each fecal sample was used for DNA extraction, using PowerSoil DNA kit (MoBio, USA) according to the manufacturer’s instructions. The KAPA HiFiHotStartReadyMix (KAPA, USA) was used to amplify the 16S rRNA (V3-V4) gene tags. Amplicons were analysed on 1.5% agarose gel electrophoresis and bands of the desired size were purified using the QIAquick Gel Extraction Kit (QIAGEN, Germany). OTHER METAGENOMIC PCR PAIRED
Processing Planned read length (bp) for mate 1: 250
Planned read length (bp) for mate 2: 250
Insert size (bp): 481
Release date2018-06-01
Run
Run Accession Release date Run Data File Information
File nameFile size (MB)
CRR024587 2018-06-01 HT01_R1.fq.gz
HT01_R2.fq.gz
3.37
3.95
SubmitterJintian Xu (xujintian@big.ac.cn)
OrganizationsBeijing Institute of Genomics, Chinese Academy of Sciences
Date submitted2017-12-20