Experiment information
Accession CRX026870
Organism Bos taurus
Title C9F
BioProject PRJCA000874
BioSample SAMC036529
Platform Illumina MiSeq
Library
Library name Construction protocol Strategy Source Selection Layout
v3-v4 region of 16S rRNA gene Total DNA of rumen fluid and the feces was extracted from individual samples using a stool DNA extraction kitTM (Qiagen, Hilden, Germany). The 16S universal primers 338 F (5?- ACTCCTACGGGAGGCAGCAG-3?) and 806 R (5?-GGACTACHVGGGTWTCTAAT-3?) were used to amplify 16S rRNA genes (regions V3-V4). Following ampli?cation, PCR products were analyzed on a 2% agarose gel to con?rm correct product size, and were purified with AxyPrep DNA Purification kit (Axygen Biosciences, Union City, USA). The purified PCR products were quantitatively determined using QuantiFluor-ST Fluoremeter (Promega, Wisconsin, USA) and PicoGreen dsDNA Quantitation Reagent (Invitrogen, Carlsbad, USA), and then were pooled in equimolar and paired-end sequenced (2×300) on an Illumina MiSeq platform (San Diego, CA, United States) according to the standard protocols. WGS METAGENOMIC PCR PAIRED
Processing Planned read length (bp) for mate 1: 250
Planned read length (bp) for mate 2: 250
Insert size (bp): 420
Release date2019-05-01
Run
Run accession Run data file information
File nameFile size (MB)
CRR029903 CRR029903_f1.fastq.gz
CRR029903_r2.fastq.gz
7.47
10.55
SubmitterShoukun Ji (jishoukun@163.com)
OrganizationChina Agricultural University
Date submitted2018-05-11