Experiment information
Accession CRX026960
Organism Mus musculus
Title scRNA-Seq_ESCs
BioProject PRJCA000878
BioSample SAMC036619
Platform Illumina HiSeq X Ten
Library
Library name Construction protocol Strategy Source Selection Layout
Cells at different timepoints throughout chemical reprogramming process were harvested and resuspended at 1 × 106 cells per milliliter in 1 × PBS with 0.04% BSA. Then, cell suspensions (300-1000 living cells per microliter determined by trypan blue staining) were loaded on a Chromium Single Cell Controller (10x Genomics) to generate single-cell gel beads in emulsion (GEMs) by using Single Cell 3' Library and Gel Bead Kit V2 (10x Genomics, 120237). Captured cells were lysed and the released RNA were barcoded through reverse transcription in individual GEMs (Zheng et al., 2017). Barcoded cDNAs were pooled and cleanup by using DynaBeads® MyOne™ Silane Beads (Invitrogen, 37002D). Single-cell RNA-seq libraries were prepared using Single Cell 3' Library Gel Bead Kit V2 (10x Genomics, 120237) following the manufacture’s introduction. RNA-Seq TRANSCRIPTOMIC PCR PAIRED
Processing Planned read length (bp) for mate 1: 26
Planned read length (bp) for mate 2: 98
Release date2018-06-25
Run
Run accession Run data file information
File nameFile size (MB)
CRR029993 CRR029993.bam 76,862.44
SubmitterYifang Liu (liuyifang3000@gmail.com)
OrganizationPeking University
Date submitted2018-05-15