Experiment information
Accession CRX027019
Organism Canis lupus familiaris
Title FAM003017.EXP1
BioProject PRJCA000879
BioSample SAMC036711
Platform Illumina HiSeq X Ten
Library
Library name Construction protocol Strategy Source Selection Layout
We used a QIAGEN DNeasy Blood & Tissue kit to extract genomic DNA and sheared to fragments randomly with the Covaris system. Then the libraries were constructed through end-repair, A-tailing, adapter ligation, and purification using the NEBNext Ultra DNA Library Prep Kit for Illumina. The concentrations of the libraries were measured using Qubit2.0. The libraries were diluted to 1.5ng/ul. Then the insert size was checked using Agilent 2100 and the purity was determined using Q-PCR method. Finally, it was sequenced on Illumina HiSeq platform. WGS GENOMIC PCR PAIRED
Processing Planned read length (bp) for mate 1: 150
Planned read length (bp) for mate 2: 150
Insert size (bp): 300
Release date2019-07-25
Run
Run Accession Release date Run Data File Information
File nameFile size (MB)
CRR030063 2019-07-25 FCHFFF7ALXX_L1_wHAXPI046819-81_1.fq.gz
FCHFFF7ALXX_L1_wHAXPI046819-81_2.fq.gz
4,689.67
5,427.31
SubmitterBo-wen Zhou (zhoubowen@mail.kiz.ac.cn)
OrganizationsKunming Institute of Zoology, Chinese Academy of Sciences
Date submitted2018-05-21