Experiment information
Accession CRX027031
Organism Canis lupus familiaris
Title FAMIKEN00001.EXP1
BioProject PRJCA000879
BioSample SAMC036716
Platform Illumina HiSeq X Ten
Library
Library name Construction protocol Strategy Source Selection Layout
We used a QIAGEN DNeasy Blood & Tissue kit to extract genomic DNA and sheared to fragments randomly with the Covaris system. Then the libraries were constructed through end-repair, A-tailing, adapter ligation, and purification using the NEBNext Ultra DNA Library Prep Kit for Illumina. The concentrations of the libraries were measured using Qubit2.0. The libraries were diluted to 1.5ng/ul. Then the insert size was checked using Agilent 2100 and the purity was determined using Q-PCR method. Finally, it was sequenced on Illumina HiSeq platform. WGS GENOMIC PCR PAIRED
Processing Planned read length (bp) for mate 1: 150
Planned read length (bp) for mate 2: 150
Insert size (bp): 325
Release date2019-07-25
Run
Run Accession Release date Run Data File Information
File nameFile size (MB)
CRR030083 2019-07-25 5041_1_clean.fq.gz
5041_2_clean.fq.gz
1,121.73
1,249.31
CRR030084 2019-07-25 5041_L6_1_clean.fq.gz
5041_L6_2_clean.fq.gz
5,484.29
6,138.39
CRR059190 2019-07-25 5041_L3_1_clean.fq.gz
5041_L3_2_clean.fq.gz
3,940.02
4,434.52
SubmitterBo-wen Zhou (zhoubowen@mail.kiz.ac.cn)
OrganizationsKunming Institute of Zoology, Chinese Academy of Sciences
Date submitted2018-05-21