Accession | CRX027032 |
Organism | Canis lupus familiaris |
Title | FAMIKEN00002.EXP1 |
BioProject | PRJCA000879 |
BioSample | SAMC036717 |
Platform | Illumina HiSeq X Ten |
Library |
Library name |
Construction protocol |
Strategy |
Source |
Selection |
Layout |
|
We used a QIAGEN DNeasy Blood & Tissue kit to extract genomic DNA and sheared to fragments randomly with the Covaris system. Then the libraries were constructed through end-repair, A-tailing, adapter ligation, and purification using the NEBNext Ultra DNA Library Prep Kit for Illumina. The concentrations of the libraries were measured using Qubit2.0. The libraries were diluted to 1.5ng/ul. Then the insert size was checked using Agilent 2100 and the purity was determined using Q-PCR method. Finally, it was sequenced on Illumina HiSeq platform. |
WGS |
GENOMIC |
PCR |
PAIRED
|
|
Processing |
Planned read length (bp) for mate 1: 150
Planned read length (bp) for mate 2: 150
Insert size (bp): 325
|
Release date | 2019-07-25 |
Run |
Run accession |
Run data file information |
File name | File size (MB) |
CRR030085 |
CRR030085_f1.fq.gz
CRR030085_r2.fq.gz
|
5,322.59
5,967.15
|
CRR030086 |
CRR030086_f1.fq.gz
CRR030086_r2.fq.gz
|
5,290.92
5,946.21
|
|
Submitter | Bo-wen Zhou (zhoubowen@mail.kiz.ac.cn) |
Organization | Kunming Institute of Zoology, Chinese Academy of Sciences |
Date submitted | 2018-05-21 |