Experiment information
Accession CRX041610
Organism Zea mays
Title maize RNA-Seq rep1
BioProject PRJCA000911
BioSample SAMC043773
Platform Illumina HiSeq X Ten
Library
Library name Construction protocol Strategy Source Selection Layout
RNA libraries were constructed according to TruSeq Stranded mRNA Sample Preparation Guide (TruSeq Stranded mRNA LT-SetA. RS-122-1201). In brief, purified poly(A)+ RNA from 2ug total RNA with RNA Purification Beads, mRNA fragmented by Fragment Prime Finish Mix. The first cDNA strand was synthesized by using First Strand Synthesis Act D Mix and SuperScript II. The second-strand was synthesized by using cDNA Second Strand Marking Master Mix, cDNA fragments was added to dATP, the Illumina PE adapters were ligated onto the ‘A’ base on repaired cDNA ends and PCR enrichment. Finally, purified DNA with AMPure XP beads . RNA-Seq GENOMIC unspecified PAIRED
Processing Planned read length (bp) for mate 1: 150
Planned read length (bp) for mate 2: 150
Insert size (bp): 400
Planned read length (bp): 150
Release date2019-06-19
Run
Run accession Run data file information
File nameFile size (MB)
CRR046362 CRR046362_f1.fq.gz
CRR046362_r2.fq.gz
2,286.55
2,411.84
Submitterdan xiong (dan-xiong@outlook.com)
OrganizationHuazhong Agricultural University
Date submitted2019-02-18