| Accession | CRX041611 |
| Organism | Zea mays |
| Title | maize RNA-Seq rep2 |
| BioProject | PRJCA000911 |
| BioSample | SAMC065451 |
| Platform | Illumina HiSeq X Ten |
| Library |
| Library name |
Construction protocol |
Strategy |
Source |
Selection |
Layout |
|
RNA libraries were constructed according to TruSeq Stranded mRNA Sample Preparation Guide (TruSeq Stranded mRNA LT-SetA. RS-122-1201). In brief, purified poly(A)+ RNA from 2ug total RNA with RNA Purification Beads, mRNA fragmented by Fragment Prime Finish Mix. The first cDNA strand was synthesized by using First Strand Synthesis Act D Mix and SuperScript II. The second-strand was synthesized by using cDNA Second Strand Marking Master Mix, cDNA fragments was added to dATP, the Illumina PE adapters were ligated onto the ‘A’ base on repaired cDNA ends and PCR enrichment. Finally, purified DNA with AMPure XP beads. |
RNA-Seq |
GENOMIC |
unspecified |
PAIRED
|
|
| Processing |
Planned read length (bp) for mate 1: 150
Planned read length (bp) for mate 2: 150
Insert size (bp): 400
|
| Release date | 2019-06-19 |
|---|
| Run |
| Run accession |
Run data file information |
| File name | File size (MB) |
|---|
| CRR046363 |
CRR046363_f1.fq.gz
CRR046363_r2.fq.gz
|
2,116.01
2,227.66
|
|
| Submitter | dan xiong (dan-xiong@outlook.com) |
|---|
| Organization | Huazhong Agricultural University |
|---|
| Date submitted | 2019-02-18 |
|---|
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