Experiment information
Accession CRX028844
Organism Ophiocordyceps sinensis
Title A comparative trancriptomic- characterization of naturally- and artificially-cultivated Ophiocordyceps sinensis
BioProject PRJCA000970
BioSample SAMC044635
Platform Illumina HiSeq 2000
Library
Library name Construction protocol Strategy Source Selection Layout
cDNA RNA was extracted from the fruiting body of the natural-growth O. sinensis, purified using magnetic beads with oligo (dT), and then interrupted into the fragments of about 200-700 nt in fragmentation buffer, which were used as templates to synthesize the first-strand cDNA using reverse transcriptase and random hexamer-primers. The constructed cDNA libraries were constructed through A-tailing, adaptor ligation and sequenced with the Illumina HiSeq 2000 using the paired-end 125bp method. RNA-Seq TRANSCRIPTOMIC PCR PAIRED
Processing Planned read length (bp) for mate 1: 100
Planned read length (bp) for mate 2: 100
Insert size (bp): 200
Planned read length (bp): 125
Release date2019-04-02
Run
Run accession Run data file information
File nameFile size (MB)
CRR032089 CRR032089_f1.fastq.gz
CRR032089_r2.fastq.gz
1,800.75
2,306.74
Submittertong xinxin (1842161947@qq.com)
OrganizationBeijing Institute of Genomics, Chinese Academy of Sciences
Date submitted2018-08-12
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