Experiment information
Accession CRX028841
Organism Homo sapiens
Title siNSUN2-BS
BioProject PRJCA000975
BioSample SAMC044839
Platform Illumina HiSeq X Ten
Library
Library name Construction protocol Strategy Source Selection Layout
Total RNA was isolated using TRIzol® Reagent (Ambion). The RNA concentration and quality were determined with NanoDrop, Qubit and agarose gel analysis. The Dynabeads® mRNA Purification Kit (Ambion) was used to enrich the mRNAs, which were used as templates for RNA-BisSeq library construction. For RNA-BisSeq, bisulfite-treated mRNAs were first subjected to reverse transcription with ACT hexamers and Superscript II Reverse Transcriptase (Invitrogen), and the following processes were carried out with KAPA mRNA Stranded mRNA-Seq Kit (KAPA) according to the manufacturer’s instructions. The libraries were sequenced on the Illumina HiSeq X-Ten platform at Novogene (Tianjin, CA) with paired-end 150 bp read length. Bisulfite-Seq TRANSCRIPTOMIC PCR PAIRED
Processing Planned read length (bp) for mate 1: 150
Planned read length (bp) for mate 2: 150
Insert size (bp): 100
Release date2019-06-11
Run
Run accession Run data file information
File nameFile size (MB)
CRR032082 CRR032082_f1.fastq.gz
CRR032082_r2.fastq.gz
12,071.08
14,333.63
Submittersun baofa (sunbf@big.ac.cn)
OrganizationBeijing Institute of Genomics, Chinese Academy of Sciences
Date submitted2018-08-10