| Accession | CRX044960 |
| Organism | Homo sapiens |
| Title | ALYREF_PARCLIP_rep2 |
| BioProject | PRJCA000975 |
| BioSample | SAMC064556 |
| Platform | Illumina HiSeq X Ten |
| Library |
| Library name |
Construction protocol |
Strategy |
Source |
Selection |
Layout |
|
The PAR-CLIP sample was separated by SDS-PAGE, RNA were recovered from the gel by D-tubeTM Dialyzer Midi(Millipore).The mixture were digested by 4 ?g/?l proteinase K (Roche, 03115828001) and precipitated in ethanol with the help of glycogen (Thermo, R0551). RNAs in ethanol were used for the small RNA library construction (NEB, E7300L). Finally, the library was sequenced on the Illumina HiSeq X-Ten platform with paired-end 150 bp (PE150) kits. |
Targeted-Capture |
TRANSCRIPTOMIC |
PCR |
SINGLE
|
|
| Processing |
Planned read length (bp): 150
|
| Release date | 2019-06-11 |
|---|
| Run |
| Run accession |
Run data file information |
| File name | File size (MB) |
|---|
| CRR049791 |
CRR049791.fq.gz
|
2,273.52
|
|
| Submitter | sun baofa (sunbf@big.ac.cn) |
|---|
| Organization | Beijing Institute of Genomics, Chinese Academy of Sciences |
|---|
| Date submitted | 2019-03-20 |
|---|
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