Experiment information
Accession CRX028913
Organism Astragalus adsurgens
Title Erect milkvetch 2
BioProject PRJCA000988
BioSample SAMC044898
Platform Illumina HiSeq 2000
Library
Library name Construction protocol Strategy Source Selection Layout
Erect milkvetch library The poly (A) mRNA was isolated by magnetic oligo (dT) beads, fragmentation buffer was added at appropriate temperatures to breaking the mRNA into short fragments. First-strand cDNA was synthesized by the interrupted mRNA as a template using random hexamer-primer. The second-strand cDNA was synthesized using buffer, dNTPs, RNaseH and DNA polymerase I. Short fragments were purified using Min Elute PCR Purification Kit (Qiagen, China), sticky-end repair and tailing A. Then, the short fragments were ligated to sequencing adapters, selected the suitable size of the fragment by the 1% agarose gel electrophoresis and performed the PCR amplification. Finally, the library was sequenced using Illumina HiSeqTM 2000 platform RNA-Seq TRANSCRIPTOMIC DNAse PAIRED
Processing Planned read length (bp) for mate 1: 150
Planned read length (bp) for mate 2: 150
Insert size (bp): 150
Release date2019-08-17
Run
Run Accession Release date Run Data File Information
File nameFile size (MB)
CRR032203 2019-08-17 2-2_1.clean.fq.gz
2-2_2.clean.fq.gz
2,022.7
2,317.74
CRR032204 2019-08-17 2-3_1.clean.fq.gz
2-3_2.clean.fq.gz
2,428.21
2,581.35
CRR032205 2019-08-17 2-1_1.clean.fq.gz
2-1_2.clean.fq.gz
2,612.59
3,081.13
Submitterzan wang (wangzan@caas.cn)
OrganizationsInstitute of Animal Science, Chinese Academy of Agricultural Sciences
Date submitted2018-08-17
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