| MOC3 ChIP-seq data of Input sample |
To conduct MOC3 ChIP-seq assay, 4 g transgenic calli expressing ACT:MOC3-GFP in Nipponbare were cross-linked in 1% formaldehyde under vacuum, and the cross-linking was stopped with 0.125 M glycine. The samples were ground to powder in liquid nitrogen, and the nuclei were isolated. Anti-GFP polyclonal antibodies (Abcam, catalog no. ab290) were used to immunoprecipitate the protein-DNA complex, and the precipitated DNA was recovered and used for the ChIP-seq assay according to the method described previously (Fu et al., 2012) with slight modifications. ChIP-seq data analysis was performed as described previously (Lu et al., 2013). |
ChIP-Seq |
GENOMIC |
ChIP |
PAIRED
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