Experiment information
Accession CRX029160
Organism Danio rerio
Title WT_0hpf_rep2_RNA-seq
BioProject PRJCA001013
BioSample SAMC045530
Platform Illumina HiSeq 2500
Library
Library name Construction protocol Strategy Source Selection Layout
Total RNA was isolated from zebrafish embryos harvested at different stages using TRIzol® Reagent (Ambion). mRNA was extracted with using Dynabeads® mRNA Purification Kit (Ambion) and subjected to TURBO™ DNase (Invitrogen) treatment at 37°C for 30 min and ethanol precipitation. Thus purified mRNA was used for RNA-Seq library construction. Libraries were directly constructed using the KAPA Stranded mRNA-Seq Kit (KAPA) and were sequenced using HiSeq2500 (Illumina) in paired-read mode, creating reads with a length of 125 bp. RNA-Seq TRANSCRIPTOMIC PCR PAIRED
Processing Planned read length (bp) for mate 1: 125
Planned read length (bp) for mate 2: 125
Insert size (bp): 240
Release date2019-06-12
Run
Run Accession Release date Run Data File Information
File nameFile size (MB)
CRR032479 2019-06-12 WT_0hpf_rep2_R1.fastq.gz
WT_0hpf_rep2_R2.fastq.gz
2,973.69
3,101.04
Submittersun baofa (sunbf@big.ac.cn)
OrganizationsBeijing Institute of Genomics, Chinese Academy of Sciences
Date submitted2018-08-31