Experiment information
Accession CRX029177
Organism Danio rerio
Title iCLIP_4hpf_rep1_Ybx1-iCLIP
BioProject PRJCA001013
BioSample SAMC045547
Platform Illumina HiSeq 2500
Library name Construction protocol Strategy Source Selection Layout
1000 zebrafish embryos at 4 hpf were irradiated twice with 0.8 J/cm2 (Stratalinker 2400, Stratagene), lysed and subjected to mild fragmentation. Crosslinked RNA-protein complexes were immunoprecipated using polyclonal Ybx1 antibody (Abmax) and protein A dynabeads. RNA was extracted and subjected to library construction using Smarter smRNA-Seq kit (Takara). All the libraries were sequenced using HiSeq2500 (Illumina) in paired-read mode, creating reads with a length of 150 bp. Targeted-Capture TRANSCRIPTOMIC PCR PAIRED
Processing Planned read length (bp) for mate 1: 150
Planned read length (bp) for mate 2: 150
Insert size (bp): 250
Release date2019-06-12
Run Accession Release date Run Data File Information
File nameFile size (MB)
CRR032496 2019-06-12 iCLIP_4hpf_rep1_R1.fastq.gz
Submittersun baofa (sunbf@big.ac.cn)
OrganizationsBeijing Institute of Genomics, Chinese Academy of Sciences
Date submitted2018-08-31