Experiment information
Accession CRX030742
Organism Danio rerio
Title MOpabpc1a_4hpf_rep1_RNA-seq
BioProject PRJCA001013
BioSample SAMC047053
Platform Illumina HiSeq 2500
Library
Library name Construction protocol Strategy Source Selection Layout
Total RNA was isolated from zebrafish embryos harvested at different stages using TRIzol® Reagent (Ambion). mRNA was extracted with using Dynabeads® mRNA Purification Kit (Ambion) and subjected to TURBO™ DNase (Invitrogen) treatment at 37°C for 30 min and ethanol precipitation. Thus purified mRNA was used for RNA-Seq library construction. Libraries were directly constructed using the KAPA Stranded mRNA-Seq Kit (KAPA) and were sequenced using HiSeq2500 (Illumina) in paired-read mode, creating reads with a length of 125 bp. RNA-Seq TRANSCRIPTOMIC PCR PAIRED
Processing Planned read length (bp) for mate 1: 125
Planned read length (bp) for mate 2: 125
Insert size (bp): 240
Release date2019-06-12
Run
Run Accession Release date Run Data File Information
File nameFile size (MB)
CRR034486 2019-06-12 MOpabpc1a_4hpf_rep1_R1.fastq.gz
MOpabpc1a_4hpf_rep1_R2.fastq.gz
4,512.68
5,031.78
Submittersun baofa (sunbf@big.ac.cn)
OrganizationsBeijing Institute of Genomics, Chinese Academy of Sciences
Date submitted2018-10-12