Accession | CRX048006 |
Organism | Danio rerio |
Title | ribo-amanitin_4hpf_rep2_RNA-seq |
BioProject | PRJCA001013 |
BioSample | SAMC067634 |
Platform | Illumina HiSeq 2500 |
Library |
Library name |
Construction protocol |
Strategy |
Source |
Selection |
Layout |
|
Synchronously developing embryos were treated with 0.2 ng of Pol II inhibition ?-amanitin (Sigma) at one-cell stage and were collected at indicated time points. 100 embryos were collected for each time point in duplicates. The total RNA was extracted by Trizol (Invitrogen) and used for RNA-Seq. An equal amount of external RNA control consortium (ERCC) RNA spike-in control (Thermo Fisher) was added to the total RNA samples as internal controls. The RNA was subjected to ribosomal RNA depletion with Ribo-Zero rRNA Removal Kit (Illumina) followed by library construction using NEB Next® Ultra™ RNA library Prep Kit (NEB). RNA stability profiling was generated from two biological replicates. |
RNA-Seq |
TRANSCRIPTOMIC |
PCR |
PAIRED
|
|
Processing |
Planned read length (bp) for mate 1: 150
Planned read length (bp) for mate 2: 150
Insert size (bp): 240
|
Release date | 2019-06-12 |
Run |
Run accession |
Run data file information |
File name | File size (MB) |
CRR053060 |
CRR053060_f1.fastq.gz
CRR053060_r2.fastq.gz
|
4,685.52
5,034.58
|
|
Submitter | sun baofa (sunbf@big.ac.cn) |
Organization | Beijing Institute of Genomics, Chinese Academy of Sciences |
Date submitted | 2019-04-12 |