Experiment information
Accession CRX048008
Organism Danio rerio
Title ribo-amanitin_6hpf_rep2_RNA-seq
BioProject PRJCA001013
BioSample SAMC067636
Platform Illumina HiSeq 2500
Library
Library name Construction protocol Strategy Source Selection Layout
Synchronously developing embryos were treated with 0.2 ng of Pol II inhibition ?-amanitin (Sigma) at one-cell stage and were collected at indicated time points. 100 embryos were collected for each time point in duplicates. The total RNA was extracted by Trizol (Invitrogen) and used for RNA-Seq. An equal amount of external RNA control consortium (ERCC) RNA spike-in control (Thermo Fisher) was added to the total RNA samples as internal controls. The RNA was subjected to ribosomal RNA depletion with Ribo-Zero rRNA Removal Kit (Illumina) followed by library construction using NEB Next® Ultra™ RNA library Prep Kit (NEB). RNA stability profiling was generated from two biological replicates. RNA-Seq TRANSCRIPTOMIC PCR PAIRED
Processing Planned read length (bp) for mate 1: 150
Planned read length (bp) for mate 2: 150
Insert size (bp): 240
Release date2019-06-12
Run
Run Accession Release date Run Data File Information
File nameFile size (MB)
CRR053062 2019-06-12 ribo-amanitin_6hpf_rep2_R1.fastq.gz
ribo-amanitin_6hpf_rep2_R2.fastq.gz
4,592.31
4,951.67
Submittersun baofa (sunbf@big.ac.cn)
OrganizationsBeijing Institute of Genomics, Chinese Academy of Sciences
Date submitted2019-04-12