Experiment information
Accession CRX036738
Organism Mus musculus
Title PriSG-A_HiC2
BioProject PRJCA001164
BioSample SAMC051444
Platform Illumina HiSeq X Ten
Library
Library name Construction protocol Strategy Source Selection Layout
The Hi-C experiment was performed follow the in-situ Hi-C protocol. Briefly, cells were lysis and chromatin was digested by MobI, then biotin fill-in and Hi-C ligation. After ligation, DNA was purified and sonicated to 200-500bp for library construction follow the . DNA was end-repaired, dATP attached and adapter ligated follow the in-situ Hi-C protocl. After adapter ligation DNA was PCR amplified and library fragments between 200-500bp were purified for sequencing. The purified library was sequenced on HiSeq X ten. Hi-C GENOMIC Restriction Digest PAIRED
Processing Planned read length (bp) for mate 1: 150
Planned read length (bp) for mate 2: 150
Insert size (bp): 300
Release date2020-12-02
Run
Run accession Run data file information
File nameFile size (MB)
CRR041308 CRR041308_f1.fastq.gz
CRR041308_r2.fastq.gz
1,731.69
2,053.26
SubmitterXiaoyuan Song (songxy5@ustc.edu.cn)
OrganizationUniversity of Science and Technology of China
Date submitted2018-12-14