Experiment information
Accession CRX036743
Organism Mus musculus
Title plpSC_HiC1
BioProject PRJCA001164
BioSample SAMC051449
Platform Illumina HiSeq X Ten
Library
Library name Construction protocol Strategy Source Selection Layout
The Hi-C experiment was performed follow the in-situ Hi-C protocol. Briefly, cells were lysis and chromatin was digested by MobI, then biotin fill-in and Hi-C ligation. After ligation, DNA was purified and sonicated to 200-500bp for library construction follow the . DNA was end-repaired, dATP attached and adapter ligated follow the in-situ Hi-C protocl. After adapter ligation DNA was PCR amplified and library fragments between 200-500bp were purified for sequencing. The purified library was sequenced on HiSeq X ten. Hi-C GENOMIC Restriction Digest PAIRED
Processing Planned read length (bp) for mate 1: 150
Planned read length (bp) for mate 2: 150
Insert size (bp): 300
Release date2020-06-01
Run
Run accession Run data file information
File nameFile size (MB)
CRR041313 CRR041313_f1.fastq.gz
CRR041313_r2.fastq.gz
10,954.64
12,103.19
SubmitterXiaoyuan Song (songxy5@ustc.edu.cn)
OrganizationUniversity of Science and Technology of China
Date submitted2018-12-14
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Experiments(5)