Accession | CRX036744 |
Organism | Mus musculus |
Title | plpSC_HiC2 |
BioProject | PRJCA001164 |
BioSample | SAMC051450 |
Platform | Illumina HiSeq X Ten |
Library |
Library name |
Construction protocol |
Strategy |
Source |
Selection |
Layout |
|
The Hi-C experiment was performed follow the in-situ Hi-C protocol. Briefly, cells were lysis and chromatin was digested by MobI, then biotin fill-in and Hi-C ligation. After ligation, DNA was purified and sonicated to 200-500bp for library construction follow the . DNA was end-repaired, dATP attached and adapter ligated follow the in-situ Hi-C protocl. After adapter ligation DNA was PCR amplified and library fragments between 200-500bp were purified for sequencing. The purified library was sequenced on HiSeq X ten. |
Hi-C |
GENOMIC |
Restriction Digest |
PAIRED
|
|
Processing |
Planned read length (bp) for mate 1: 150
Planned read length (bp) for mate 2: 150
Insert size (bp): 300
|
Release date | 2020-06-01 |
Run |
Run accession |
Run data file information |
File name | File size (MB) |
CRR041314 |
CRR041314_f1.fastq.gz
CRR041314_r2.fastq.gz
|
2,275.82
2,438.9
|
|
Submitter | Xiaoyuan Song (songxy5@ustc.edu.cn) |
Organization | University of Science and Technology of China |
Date submitted | 2018-12-14 |