| Accession | CRX029738 |
| Organism | Homo sapiens |
| Title | input-Chip |
| BioProject | PRJCA001025 |
| BioSample | SAMC046090 |
| Platform | Illumina HiSeq 4000 |
| Library |
| Library name |
Construction protocol |
Strategy |
Source |
Selection |
Layout |
|
1. Pipeline of ChIP Enrichment
Cross-liking Chromatin Immunoprecitation (X- ChIP ):
(1) DNA-protein cross-linking by formaldehyde.
(2) Add Lysis Buffer to get whole cell lysate.
(3) Lysate sonication (100-500bp).
(4) Immunoprecipitation: Add primary antibody and beads to samples.
(5) Elution and reverse cross-links.
(6) qPCR for Quality Control.
(7) Ready for ChIP -seq library construction.
Native Chromatin Immunoprecipitation (N- ChIP ):
(1) Preparation of native chromatin (nuclear lysate).
(2) Micrococcal nuclease digestion.
(3) Immunoprecipitation: Add primary antibody and beads separately to samples.
(4) DNA isolation.
(5) qPCR for Quality Control.
(6) Ready for ChIP -seq library construction.
2. Pipeline of Library Preparation
(1) DNA-end repair, 3'-dA overhang and ligation of methylated sequencing adaptor.
(2) PCR amplification and size selection (usually 100-300bp, including adaptor sequence).
(3) Qualified library for sequencing. |
ChIP-Seq |
GENOMIC |
ChIP |
SINGLE
|
|
| Processing |
Planned read length (bp): 49
|
| Release date | 2019-01-05 |
|---|
| Run |
| Run accession |
Run data file information |
| File name | File size (MB) |
|---|
| CRR033172 |
CRR033172.fastq.gz
|
652.26
|
|
| Submitter | Feifei Hu (hufeifei@hust.edu.cn) |
|---|
| Organization | Huazhong University of Science and Technology |
|---|
| Date submitted | 2018-09-13 |
|---|
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