Experiment information
Accession CRX030111
Organism Arabidopsis thaliana
Title Col-0-22-1 Arabidopsis
BioProject PRJCA001018
BioSample SAMC046465
Platform Illumina HiSeq 2500
Library name Construction protocol Strategy Source Selection Layout
3µg of total RNA was used for isolating poly(A) mRNA with Poly(A) mRNA Magnetic Isolation Module (NEB). Following purification, the mRNA was fragmented into small pieces by adding fragmentation buffer. Then, the cleaved RNA fragments were reverse-transcribed to create the final cDNA library in accordance with the protocol for the NEBNext Ultua RNA Library Prep Kit (NEB). The average insert size for the paired-end libraries was 300 bp. Then, paired-end sequencing (100 bp) was carried out on the HiSeq2500 platform (Illumina) RNA-Seq TRANSCRIPTOMIC PolyA PAIRED
Processing Planned read length (bp) for mate 1: 150
Planned read length (bp) for mate 2: 150
Insert size (bp): 400
Release date2018-09-24
Run Accession Release date Run Data File Information
File nameFile size (MB)
CRR033545 2018-09-24 Col-0-22-1_combined_R1.fastq.gz
Submitterhuanhuan Jin (1152225800@qq.com)
OrganizationsNanjing Normal University
Date submitted2018-09-21
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