| Library |
| Library name |
Construction protocol |
Strategy |
Source |
Selection |
Layout |
|
A single cell was lysed with 200 ng of the carrier RNA mixture (containing 100 ng 5’-phosphorylated small carrier RNA mimic). The poly-A RNAs were selected using a NEBNext Poly(A) mRNA Magnetic Isolation Module (NEB #E7490), and the small RNAs remained in the supernatant after poly-A selection. The small RNA sequencing library was constructed following the manufacturer's protocol using a NEBNext kit (NEB #E7300). After PCR amplification (15 cycles), the cDNA fragment from the RNA carrier was removed by Not I digestion. After Not I digestion, the library DNA was purified for deep sequencing. |
ncRNA-Seq |
TRANSCRIPTOMIC |
other |
SINGLE
|
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