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Experiment information
Accession CRX030279
Organism Mus musculus
Title mESC_bulk_Cas9
BioProject PRJCA001042
BioSample SAMC046631
Platform Illumina HiSeq 2500
Library
Library name Construction protocol Strategy Source Selection Layout
Total RNA was extracted from approximately one million mESCs using a miRNeasy Micro kit (QIAGEN). The libraries were constructed following the manufacturer's protocol using NEBNext kits (E7490&E7530), before PCR enrichment, the DNA was digested by Cas9 nuclease with sgRNA mix at 37 degree Celsius for 2h RNA-Seq TRANSCRIPTOMIC PolyA SINGLE
Processing Planned read length (bp): 50
Release date2018-09-30
Run
Run accession Run data file information
File nameFile size (MB)
CRR033727 CRR033727.fastq.gz 652.99
SubmitterZhengyun Xiao (xiaozhengyun1993@126.com)
OrganizationZhejiang University
Date submitted2018-09-23
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