Accession | CRX030284 |
Organism | Mus musculus |
Title | mESC_smart_1ng_Nextera_2 |
BioProject | PRJCA001042 |
BioSample | SAMC046626 |
Platform | Illumina HiSeq 2500 |
Library |
Library name |
Construction protocol |
Strategy |
Source |
Selection |
Layout |
|
cDNA from 1 ng mESC total RNA was produced following the manufacturer's protocol using the Smart-Seq2 v4 Ultra Low Input RNA kit (Clontech) for single cells. Then, 1 ng of cDNA was used to construct the sequencing library using illumina Nextera XT DNA library preparation kit (FC-131-1024). |
RNA-Seq |
TRANSCRIPTOMIC |
PolyA |
SINGLE
|
|
Processing |
Planned read length (bp): 50
|
Release date | 2018-09-30 |
Run |
Run accession |
Run data file information |
File name | File size (MB) |
CRR033722 |
CRR033722.fastq.gz
|
751.7
|
|
Submitter | Zhengyun Xiao (xiaozhengyun1993@126.com) |
Organization | Zhejiang University |
Date submitted | 2018-09-23 |