Experiment information
Accession CRX030205
Organism Homo sapiens
Title 293T_total_sc2
BioProject PRJCA001042
BioSample SAMC046550
Platform Illumina HiSeq 2500
Library
Library name Construction protocol Strategy Source Selection Layout
A single cell was lysed with 100 ng of the carrier RNA mixture. The sequencing library was constructed following the manufacturer’s protocol using a NEBNext kit (E7530). After adaptor ligation and USER digestion, cDNA fragments from the RNA carrier were removed by Not I digestion. After Not I digestion, the library DNA was purified for PCR amplification. RNA-Seq TRANSCRIPTOMIC RANDOM FRAGMENT
Processing Planned read length (bp): 50
Release date2018-09-29
Run
Run Accession Release date Run Data File Information
File nameFile size (MB)
CRR033646 2018-09-29 293T_total_sc2.fastq.gz 563.37
SubmitterZhengyun Xiao (xiaozhengyun1993@126.com)
OrganizationsZhejiang University
Date submitted2018-09-23