Experiment information
Accession CRX030211
Organism Homo sapiens
Title BreastCancerSC_afterCapt
BioProject PRJCA001042
BioSample SAMC046556
Platform Illumina HiSeq 2500
Library
Library name Construction protocol Strategy Source Selection Layout
A single cell was lysed with 100 ng of the carrier RNA mixture. The sequencing library was constructed following the manufacturer’s protocol using a NEBNext kit (E7420). After adaptor ligation, the cDNA fragments from the RNA carrier were removed by Not I digestion. After Not I digestion, the library DNA was purified for USER digestion and PCR amplification. PCR amplification was performed using primers containing 8-nt degenerate barcodes to perform the first 10 cycles of library amplification, followed by another 15 cycles of amplification by regular P5/P7 primers. The directional Holo-Seq library of total RNA was used for target capturing using the Illumina TruSeq Exome Capture kit (FC-144-1004). Targeted-Capture TRANSCRIPTOMIC padlock probes capture method FRAGMENT
Processing Planned read length (bp): 50
Release date2018-09-29
Run
Run Accession Release date Run Data File Information
File nameFile size (MB)
CRR033652 2018-09-29 BreastCancerSC_afterCapt.fastq.gz 256.63
SubmitterZhengyun Xiao (xiaozhengyun1993@126.com)
OrganizationsZhejiang University
Date submitted2018-09-23