Experiment information
Accession CRX030224
Organism Homo sapiens
Title HCC_dual_mRNA_13
BioProject PRJCA001042
BioSample SAMC046569
Platform Illumina HiSeq 2500
Library
Library name Construction protocol Strategy Source Selection Layout
Resected tumor sample was collected from a 46-year-old male patient and transported in DMEM (GIBCO) on ice immediately after surgical. Tumor pieces were digested with accumax to obtain single cell suspension. Single cells were picked up and lysed with 200 ng carrier RNA mixture (containing 100 ng 5’-phosphorylated small carrier RNA mimic). The poly-A RNAs were selected using a NEBNext Poly(A) mRNA Magnetic Isolation Module (NEB #E7490), and the small RNAs remained in the supernatant after poly-A selection. The poly(A) mRNA sequencing library was constructed following the manufacturer’s protocol using a NEBNext kit (NEB #E7530). After adaptor ligation and USER digestion, cDNA fragments from the RNA carrier were removed by Not I digestion. After Not I digestion, the library DNA was purified for PCR amplification. RNA-Seq TRANSCRIPTOMIC PolyA FRAGMENT
Processing Planned read length (bp): 50
Release date2018-09-29
Run
Run Accession Release date Run Data File Information
File nameFile size (MB)
CRR033665 2018-09-29 HCC_dual_mRNA_13.fastq.gz 21.55
SubmitterZhengyun Xiao (xiaozhengyun1993@126.com)
OrganizationsZhejiang University
Date submitted2018-09-23