Experiment information
Accession CRX030292
Organism Homo sapiens
Title 293T_bulk_Not1
BioProject PRJCA001042
BioSample SAMC046633
Platform Illumina HiSeq 2500
Library
Library name Construction protocol Strategy Source Selection Layout
Total RNA was extracted from approximately one million HEK293T cells using a miRNeasy Micro kit (QIAGEN). The libraries were constructed following the manufacturer's protocol using NEBNext kits (E7490&E7530), before PCR enrichment, the DNA was digested by Not1 at 37 degree Celsius for 2h RNA-Seq TRANSCRIPTOMIC PolyA FRAGMENT
Processing Planned read length (bp): 50
Release date2018-09-29
Run
Run Accession Release date Run Data File Information
File nameFile size (MB)
CRR033729 2018-09-29 293T_bulk_Not1.fastq.gz 613.22
SubmitterZhengyun Xiao (xiaozhengyun1993@126.com)
OrganizationsZhejiang University
Date submitted2018-09-23