Experiment information
Accession CRX030293
Organism Homo sapiens
Title 293T_bulk_Cas9
BioProject PRJCA001042
BioSample SAMC046634
Platform Illumina HiSeq 2500
Library
Library name Construction protocol Strategy Source Selection Layout
Total RNA was extracted from approximately one million HEK293T cell using a miRNeasy Micro kit (QIAGEN).The libraries were constructed following the manufacturer's protocol using NEBNext kits (E7490&E7530),before PCR enrichment, the DNA was digested by Cas9 nuclease with sgRNA mix at 37 degree Celsius for 2h. RNA-Seq TRANSCRIPTOMIC PolyA FRAGMENT
Processing Planned read length (bp): 50
Release date2018-09-29
Run
Run Accession Release date Run Data File Information
File nameFile size (MB)
CRR033728 2018-09-29 293T_bulk_Cas9.fastq.gz 647.01
SubmitterZhengyun Xiao (xiaozhengyun1993@126.com)
OrganizationsZhejiang University
Date submitted2018-09-23