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Experiment information
Accession CRX030668
Organism Oryza sativa
Title TGS for the full-length mRNA sequences - 2-3kb
BioProject PRJCA001057
BioSample SAMC046965
Platform PacBio RS II
Library
Library name Construction protocol Strategy Source Selection Layout
2-3kb Following isolation, purification and determination were performed using the method mentioned in the SGS process, and the total RNA of the combined sample was reversed-transcribed into first-strand cDNA using a SMARTer PCR cDNA Synthesis Kit (Clontech Laboratories, USA). The cDNA was then amplified by large-scale PCR for 10 cycles, column-purified, and then size-selected into cDNA fractions in sizes of 1-2 kb, 2-3 kb, and 3-6 kb using a BluePippin System (Sage Science, USA). Two SMRT cells for the 1-2 kb fractions, two for the 2-3 kb fractions, and one for the 3-6 kb fractions were run on the PacBio RS II platform for sequencing. FL-cDNA TRANSCRIPTOMIC RT-PCR SINGLE
Processing
Release date2018-10-17
Run
Run accession Run data file information
File nameFile size (MB)
CRR034190 CRR034190.fastq.gz 890.72
CRR034450 CRR034450.fastq.gz 844.46
CRR034451 CRR034451.fastq.gz 846.36
CRR034452 CRR034452.fastq.gz 521.76
CRR034453 CRR034453.fastq.gz 514.39
CRR034454 CRR034454.fastq.gz 528.3
SubmitterLiao Jiang-Lin (jlliao514815@163.com)
OrganizationJiangxi Agricultural University
Date submitted2018-10-07
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