Experiment information
Accession CRX030668
Organism Oryza sativa
Title TGS for the full-length mRNA sequences - 2-3kb
BioProject PRJCA001057
BioSample SAMC046965
Platform PacBio RS II
Library
Library name Construction protocol Strategy Source Selection Layout
2-3kb Following isolation, purification and determination were performed using the method mentioned in the SGS process, and the total RNA of the combined sample was reversed-transcribed into first-strand cDNA using a SMARTer PCR cDNA Synthesis Kit (Clontech Laboratories, USA). The cDNA was then amplified by large-scale PCR for 10 cycles, column-purified, and then size-selected into cDNA fractions in sizes of 1-2 kb, 2-3 kb, and 3-6 kb using a BluePippin System (Sage Science, USA). Two SMRT cells for the 1-2 kb fractions, two for the 2-3 kb fractions, and one for the 3-6 kb fractions were run on the PacBio RS II platform for sequencing. FL-cDNA TRANSCRIPTOMIC RT-PCR FRAGMENT
Processing
Release date2018-10-17
Run
Run Accession Release date Run Data File Information
File nameFile size (MB)
CRR034190 2018-10-17 Full_length_mRNA_sequencing_2-3K_cell_1_01.fastq.gz 890.72
CRR034450 2018-10-17 Full_length_mRNA_sequencing_2-3K_cell_1_02.fastq.gz 844.46
CRR034451 2018-10-17 Full_length_mRNA_sequencing_2-3K_cell_1_03.fastq.gz 846.36
CRR034452 2018-10-17 Full_length_mRNA_sequencing_2-3K_cell_2_01.fastq.gz 521.76
CRR034453 2018-10-17 Full_length_mRNA_sequencing_2-3K_cell_2_02.fastq.gz 514.39
CRR034454 2018-10-17 Full_length_mRNA_sequencing_2-3K_cell_2_03.fastq.gz 528.3
SubmitterLiao Jiang-Lin (jlliao514815@163.com)
OrganizationsJiangxi Agricultural University
Date submitted2018-10-07