Experiment information
Accession CRX030669
Organism Oryza sativa
Title TGS for the full-length mRNA sequences - 3-6kb
BioProject PRJCA001057
BioSample SAMC046965
Platform PacBio RS II
Library
Library name Construction protocol Strategy Source Selection Layout
3-6kb Following isolation, purification and determination were performed using the method mentioned in the SGS process, and the total RNA of the combined sample was reversed-transcribed into first-strand cDNA using a SMARTer PCR cDNA Synthesis Kit (Clontech Laboratories, USA). The cDNA was then amplified by large-scale PCR for 10 cycles, column-purified, and then size-selected into cDNA fractions in sizes of 1-2 kb, 2-3 kb, and 3-6 kb using a BluePippin System (Sage Science, USA). Two SMRT cells for the 1-2 kb fractions, two for the 2-3 kb fractions, and one for the 3-6 kb fractions were run on the PacBio RS II platform for sequencing. FL-cDNA TRANSCRIPTOMIC RT-PCR FRAGMENT
Processing
Release date2018-10-17
Run
Run Accession Release date Run Data File Information
File nameFile size (MB)
CRR034191 2018-10-17 Full_length_mRNA_sequencing_3-6K_cell_1_01.fastq.gz 684.28
CRR034455 2018-10-17 Full_length_mRNA_sequencing_3-6K_cell_1_02.fastq.gz 697.21
CRR034456 2018-10-17 Full_length_mRNA_sequencing_3-6K_cell_1_03.fastq.gz 734.44
SubmitterLiao Jiang-Lin (jlliao514815@163.com)
OrganizationsJiangxi Agricultural University
Date submitted2018-10-07