Experiment information
Accession CRX030686
Organism Oryza sativa
Title XN0437T_Genome_5kb
BioProject PRJCA001057
BioSample SAMC046978
Platform Illumina HiSeq 2500
Library name Construction protocol Strategy Source Selection Layout
XN0437T_Genome_5kb Fourteen days after germination, young leaves were harvested from HTS and HSS and used for genomic DNA isolation using a DNeasy Plant Mini Kit (Qiagen, Germany) according to the manufacturer's instructions. After quality assessment by agarose gel electrophoresis and Nanodrop (Nanodrop Technologies, Wilmington, DE, US) and quantification by a Qubit fluorometer (Thermo Fisher Scientific, Waltham, MA), the DNA was used to construct sequencing libraries by SeqCap EZ Choice Library (Roche NimbleGen, USA). Six libraries of 170 bp, 500 bp, 800 bp, 2 kb, 5 kb, and 10 kb were constructed. After the library quality was assessed by an Agilent2100 Bioanalyzer, a paired-end sequencing strategy was used on the Illumina HiSeq 2500 platform. WGS GENOMIC PCR PAIRED
Processing Planned read length (bp) for mate 1: 101
Planned read length (bp) for mate 2: 101
Insert size (bp): 200
Release date2018-10-17
Run Accession Release date Run Data File Information
File nameFile size (MB)
CRR034390 2018-10-17 XN0437T_Genome_5kb_L001_R1_001.fastq.gz
SubmitterLiao Jiang-Lin (jlliao514815@163.com)
OrganizationsJiangxi Agricultural University
Date submitted2018-10-09