Experiment information
Accession CRX030735
Organism Scophthalmus maximus
Title SBM
BioProject PRJCA001060
BioSample SAMC047047
Platform Ion Torrent PGM
Library
Library name Construction protocol Strategy Source Selection Layout
The bacterial DNA from intestinal mucosa samples was extracted using the QIAamp DNA Stool Mini Kit. Then, DNA was diluted to 1 ng/μL using sterile water and 16S rRNA genes of distinct regions (16SV4) were amplified using specific primer (515f/806r) with the barcode. PCR products from all samples were mixed in equidensity ratios and purified using the GeneJETTM Gel Extraction Kit. Sequencing libraries were generated using Ion Plus Fragment Library Kit 48 rxns. The library quality was evaluated on the Qubit@ 2.0 Fluorometer. Finally, the library was sequenced on an Ion S5TM XL platform and 400 bp/600 bp single-end reads were generated. AMPLICON GENOMIC PCR FRAGMENT
Processing
Release date2018-10-18
Run
Run Accession Release date Run Data File Information
File nameFile size (MB)
CRR034476 2018-10-18 SBM1.fastq.gz 13.9
CRR034477 2018-10-18 SBM2.fastq.gz 16.23
CRR034478 2018-10-18 SBM3.fastq.gz 11.6
SubmitterChaoqun Li (1148072849@qq.com)
OrganizationsOcean University of China
Date submitted2018-10-10
Related experiments
Experiments(2)