Experiment information
Accession CRX030736
Organism Scophthalmus maximus
Title FSBM
BioProject PRJCA001060
BioSample SAMC047048
Platform Ion Torrent PGM
Library
Library name Construction protocol Strategy Source Selection Layout
The bacterial DNA from intestinal mucosa samples was extracted using the QIAamp DNA Stool Mini Kit. Then, DNA was diluted to 1 ng/μL using sterile water and 16S rRNA genes of distinct regions (16SV4) were amplified using specific primer (515f/806r) with the barcode. PCR products from all samples were mixed in equidensity ratios and purified using the GeneJETTM Gel Extraction Kit. Sequencing libraries were generated using Ion Plus Fragment Library Kit 48 rxns. The library quality was evaluated on the Qubit@ 2.0 Fluorometer. Finally, the library was sequenced on an Ion S5TM XL platform and 400 bp/600 bp single-end reads were generated. AMPLICON GENOMIC PCR SINGLE
Processing
Release date2018-10-18
Run
Run accession Run data file information
File nameFile size (MB)
CRR034479 CRR034479.fastq.gz 13.47
CRR034480 CRR034480.fastq.gz 9.21
CRR034481 CRR034481.fastq.gz 13.81
SubmitterChaoqun Li (1148072849@qq.com)
OrganizationOcean University of China
Date submitted2018-10-10
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