Experiment information
Accession CRX032190
Organism Mus musculus
Title Simulated microgravity condition
BioProject PRJCA000869
BioSample SAMC030009
Platform Illumina HiSeq 3000
Library
Library name Construction protocol Strategy Source Selection Layout
The Lin- HPCs were cultured in RPMI-1640 medium containing 10ng/ml interlelukin-3, 20ng/ml interleukin-6 and 20ng/ml stem cell factor (SCF), on rotary cell culture system (RCCS) (breathable but impermeable to liquid) with 12 rpm rotation speed and automatic replacement of 5ml medium every day, in a humidified 37°C incubator with 5% CO2 for 12 days. RNA-Seq TRANSCRIPTOMIC cDNA PAIRED
Processing Planned read length (bp) for mate 1: 150
Planned read length (bp) for mate 2: 150
Insert size (bp): 300
Release date2019-05-03
Run
Run accession Run data file information
File nameFile size (MB)
CRR036070 CRR036070_f1.fq.gz
CRR036070_r2.fq.gz
1,938.49
2,335.69
SubmitterLu Shi (shilu@ioz.ac.cn)
OrganizationInstitute of Zoology, Chinese Academy of Sciences
Date submitted2018-10-31
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Experiments(3)