Experiment information
Accession CRX032038
Organism Rattus norvegicus
BioProject PRJCA001099
BioSample SAMC047721
Platform Illumina HiSeq X-10
Library
Library name Construction protocol Strategy Source Selection Layout
Libraries for sequencing were constructed with NEB Next Ultra RNA Library Prep Kit for Illumina (NEB). Poly(A) tailed mRNA molecules were enriched from13 ?g total RNA with NEB Next Poly(A) mRNA Magnetic Isolation Module (NEB) kit.The mRNA was fragmented into approximately 200 base pair pieces. The first-strand cDNA was synthesized from the mRNA fragments reverse transcriptase and random hexamer primers, and then the second-strand cDNA was synthesized using DNA polymerase I and RNaseH. The end of the cDNA fragment was subjected to an end repair process that included the addition of a single ‘A’ base, followed by ligation of the adapters. Products were purified and enriched by polymerase chain reaction (PCR) to amplify the library DNA. The final libraries were quantified using KAPA Library Quantification Kit (KAPA Biosystems, South Africa) and an Agilent 2100 Bioanalyzer. After quantitative reverse transcription-polymerase chain reaction (RT-qPCR) validation, libraries were subjected to paired-end sequencing with 150-base pair read length on the Illumina HiSeq X ten sequencer. RNA-Seq TRANSCRIPTOMIC PCR PAIRED
Processing Planned read length (bp) for mate 1: 150
Planned read length (bp) for mate 2: 150
Insert size (bp): 200
Release date2018-11-01
Run
Run Accession Release date Run Data File Information
File nameFile size (MB)
CRR035918 2018-11-01 KB6_1.fq.gz
KB6_2.fq.gz
1,902.53
2,049.13
SubmitterXizhan Xu (xuxz@im.ac.cn)
OrganizationsInstitute of Microbiology, Chinese Academy of Sciences
Date submitted2018-10-31