| 实验编号 | CRX032038 |
| 物种名称 | Rattus norvegicus |
| 项目编号 | PRJCA001099 |
| 样本编号 | SAMC047721 |
| 测序平台 | Illumina HiSeq X Ten |
| 建库信息 |
| 文库名称 |
文库构建方法 |
建库策略 |
样品来源 |
实验选择 |
文库布局 |
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Libraries for sequencing were constructed with NEB Next Ultra RNA Library Prep Kit for Illumina (NEB). Poly(A) tailed mRNA molecules were enriched from13 ?g total RNA with NEB Next Poly(A) mRNA Magnetic Isolation Module (NEB) kit.The mRNA was fragmented into approximately 200 base pair pieces. The first-strand cDNA was synthesized from the mRNA fragments reverse transcriptase and random hexamer primers, and then the second-strand cDNA was synthesized using DNA polymerase I and RNaseH. The end of the cDNA fragment was subjected to an end repair process that included the addition of a single ‘A’ base, followed by ligation of the adapters. Products were purified and enriched by polymerase chain reaction (PCR) to amplify the library DNA. The final libraries were quantified using KAPA Library Quantification Kit (KAPA Biosystems, South Africa) and an Agilent 2100 Bioanalyzer. After quantitative reverse transcription-polymerase chain reaction (RT-qPCR) validation, libraries were subjected to paired-end sequencing with 150-base pair read length on the Illumina HiSeq X ten sequencer. |
RNA-Seq |
TRANSCRIPTOMIC |
PCR |
PAIRED
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|
| 处理信息 |
Planned read length (bp) for mate 1: 150
Planned read length (bp) for mate 2: 150
Insert size (bp): 200
|
| 发布日期 | 2018-11-01 |
|---|
| 测序反应 |
| Run编号 |
发布日期 |
Run序列文件信息 |
| File name | File size (MB) |
|---|
| CRR035918 |
2018-11-01 |
KB6_1.fq.gz
KB6_2.fq.gz
|
1,902.53
2,049.13
|
|
| 提交 | Xizhan Xu (xuxz@im.ac.cn) |
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| 所属单位 | Institute of Microbiology, Chinese Academy of Sciences |
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| 提交日期 | 2018-10-31 |
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