实验基本信息
实验编号 CRX032038
物种名称 Rattus norvegicus
项目编号 PRJCA001099
样本编号 SAMC047721
测序平台 Illumina HiSeq X Ten
建库信息
文库名称 文库构建方法 建库策略 样品来源 实验选择 文库布局
Libraries for sequencing were constructed with NEB Next Ultra RNA Library Prep Kit for Illumina (NEB). Poly(A) tailed mRNA molecules were enriched from13 ?g total RNA with NEB Next Poly(A) mRNA Magnetic Isolation Module (NEB) kit.The mRNA was fragmented into approximately 200 base pair pieces. The first-strand cDNA was synthesized from the mRNA fragments reverse transcriptase and random hexamer primers, and then the second-strand cDNA was synthesized using DNA polymerase I and RNaseH. The end of the cDNA fragment was subjected to an end repair process that included the addition of a single ‘A’ base, followed by ligation of the adapters. Products were purified and enriched by polymerase chain reaction (PCR) to amplify the library DNA. The final libraries were quantified using KAPA Library Quantification Kit (KAPA Biosystems, South Africa) and an Agilent 2100 Bioanalyzer. After quantitative reverse transcription-polymerase chain reaction (RT-qPCR) validation, libraries were subjected to paired-end sequencing with 150-base pair read length on the Illumina HiSeq X ten sequencer. RNA-Seq TRANSCRIPTOMIC PCR PAIRED
处理信息 Planned read length (bp) for mate 1: 150
Planned read length (bp) for mate 2: 150
Insert size (bp): 200
发布日期2018-11-01
测序反应
Run编号 发布日期 Run序列文件信息
File nameFile size (MB)
CRR035918 2018-11-01 KB6_1.fq.gz
KB6_2.fq.gz
1,902.53
2,049.13
提交Xizhan Xu (xuxz@im.ac.cn)
所属单位Institute of Microbiology, Chinese Academy of Sciences
提交日期2018-10-31