Experiment information
Accession CRX032485
Organism Mus musculus
Title MEF
BioProject PRJCA001125
BioSample SAMC048028
Platform Illumina HiSeq 4000
Library
Library name Construction protocol Strategy Source Selection Layout
Total RNA was extracted from cultured cells by TRIzol reagent. For RNA-seq library construction, the PolyA+ tailed RNA purification was performed for each sample using a Dynabead mRNA purification kit. The cDNA library was generated with a KAPA stranded mRNA-Seq Kit protocol. Sequencing was performed on an Illumina HiSeq 4000 platform, with 150 bp paired-end-sequencing reactions. RNA-Seq GENOMIC PolyA PAIRED
Processing Planned read length (bp) for mate 1: 125
Planned read length (bp) for mate 2: 125
Insert size (bp): 300
Release date2019-01-10
Run
Run Accession Release date Run Data File Information
File nameFile size (MB)
CRR037078 2019-01-10 MEF_R1.fastq.gz
MEF_R2.fastq.gz
2,667.46
2,659.36
SubmitterGuihai Feng (fenggh@aliyun.com)
OrganizationsInstitute of Zoology, Chinese Academy of Sciences
Date submitted2018-11-17
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