Experiment information
Accession CRX032422
Organism Apis mellifera
Title honey bee worker blastoderm methylome rep1
BioProject PRJCA000098
BioSample SAMC047970
Platform Illumina HiSeq 2000
Library
Library name Construction protocol Strategy Source Selection Layout
Genomic DNA spiked with 0.5? unmethylated Lambda DNA (Promega) was sonicated into 100-500bp. Fragmented DNA was then subjected to end repair,dA tailing, ligation with the end repair enzyme mix (NEB), Klenow 3´-5´ exo- (NEB) and T4 DNA ligase (NEB), respectively. Adapter-ligated DNA was recovered by 2? agarose gel electrophoresis, and bisulfite conversion was performed using the EZ DNA methylation-Gold kit (Zymo Research) according to the instruction manual. Bisulfite-treated DNA was amplified with KAPA HiFi HotStart Uracil+ ReadyMix. Amplified DNA was recovered by 2? agarose gel electrophoresis. Bisulfite-Seq GENOMIC RANDOM PAIRED
Processing Planned read length (bp) for mate 1: 100
Planned read length (bp) for mate 2: 100
Insert size (bp): 300
Release date2019-05-30
Run
Run Accession Release date Run Data File Information
File nameFile size (MB)
CRR036550 2019-05-30 honey_bee_worker_blastoderm_rep1_run1_1.fastq.gz
honey_bee_worker_blastoderm_rep1_run1_2.fastq.gz
2,621.32
2,471.2
Submitterxiaocui xu (xuxc@big.ac.cn)
OrganizationsBeijing Institute of Genomics, Chinese Academy of Sciences
Date submitted2018-11-13